Research Articles
Harnessing Electric Fields: The Catalytic Power of Ketosteroid Isomerase (KSI) in Enzyme Mechanism and Drug Design
This article explores the role of preorganized electric fields in ketosteroid isomerase (KSI) catalysis, a paradigm for understanding enzymatic rate enhancement.
IMS-MS Validation of Enzyme Folding in Organic Solvents: A Cutting-Edge Method for Biocatalyst and Drug Discovery
This article provides a comprehensive guide for researchers on employing Ion Mobility Spectrometry-Mass Spectrometry (IMS-MS) to validate and characterize enzyme folding in organic solvents.
His-Tag vs HaloTag vs Strep-Tag: A 2024 Guide to Optimal Protein Immobilization for Research
This article provides a comprehensive, up-to-date comparison of His-tag, HaloTag, and Strep-tag technologies for protein immobilization, crucial for assays, biosensors, and drug discovery.
Advanced Synthetic Biology: Engineering HaloTag-SNAPTag Fusion Proteins for Precision Artificial Metalloenzyme Scaffolding
This article provides a comprehensive guide for researchers on the strategic design and application of HaloTag-SNAPTag fusion proteins as versatile, dual-covalent scaffolds for Artificial Metalloenzymes (ArMs).
HaloTag Technology: Revolutionizing Biopharmaceutical Manufacturing with Covalent Immobilization in Packed Bed Reactors
This article provides a comprehensive guide to leveraging HaloTag technology for the covalent, site-specific immobilization of biocatalysts in packed bed reactors (PBRs).
Mastering Chiral Amine Analysis: A Comprehensive Guide to HPLC & SFC Enantiomeric Separation
This comprehensive guide explores the critical techniques of High-Performance Liquid Chromatography (HPLC) and Supercritical Fluid Chromatography (SFC) for the enantiomeric separation of chiral amines, essential in modern drug development.
Gene Surfing for Enzyme Discovery: A Metagenomic Workflow for Drug Development
This article provides a comprehensive guide to the Gene Surfing workflow, a computational method for mining vast metagenomic datasets to discover novel enzymes with therapeutic potential.
GDT-TS vs RMSD: Choosing the Right Metric for Protein Structure Validation and Drug Discovery
This article provides a comprehensive analysis of two fundamental metrics for protein structure validation: Global Distance Test Total Score (GDT-TS) and Root Mean Square Deviation (RMSD).
Evolution in Motion: Choosing Between FACS Screening and Microtiter Plates for Directed Enzyme Engineering
Directed evolution remains a cornerstone of enzyme engineering for biocatalysis and therapeutic development.
FACS for Enzyme Evolution: High-Throughput Screening Strategies to Accelerate Protein Engineering
This article provides a comprehensive guide for researchers on implementing Fluorescence-Activated Cell Sorting (FACS) as a high-throughput screening method for directed enzyme evolution.